Journal: Immunity

Article Title: PIP5 Kinases Regulate Membrane Phosphoinositide and Actin Composition for Targeted Granule Secretion by Cytotoxic Lymphocytes

doi: 10.1016/j.immuni.2018.08.017

Figure Lengend Snippet:

Article Snippet: SIINFEKL (Ovalbumin peptide 257-264) , Anaspec , AS-60193-1.

Techniques: Blocking Assay, Control, Virus, Recombinant, Electron Microscopy, Fractionation, Software

Journal:

Article Title: In vivo efficacy of a chitosan/IL-12 adjuvant system for protein-based vaccines

doi: 10.1016/j.biomaterials.2010.09.058

Figure Lengend Snippet: Chitosan/OVA/IL-12 stimulates IFN-γ-producing CD8+ T cells. Mice (n = 5) were vaccinated on days 0 and 14 and sacrificed on day 21. Splenocytes were pooled and cultured for 6 days with 1 μg/mL OVA257-264 (SIINFEKL) MHC class I peptide, then rested overnight in plain medium. Splenocytes (500,000 cells in 2 mL) were subsequently cultured with irradiated APCs and 1 μg/mL OVA257-264 (SIINFEKL) or control (VSV-NP) peptide for 24 h, and released IFN-γ was measured by ELISA. Results are plotted as the mean of duplicate wells.

Article Snippet: The following reagents were purchased from the suppliers indicated: ß-gal (BG13, Prozyme; Hayward, CA); FITC-, PE-, or PerCP-Cy5.5-labeled antibodies to CD8, CD3 (BD Biosciences; San Jose, CA, and eBioscience; San Diego, CA); PE-labeled MHC class I pentamers with H-2K b -restricted peptides for OVA (aa 257-264, SIINFEKL) and HSV (aa 498-505, SSIEFARL) (ProImmune; Bradenton, FL); horseradish peroxidase-conjugated goat anti-mouse IgG total, IgG1, IgG2a, and IgG2b antibodies (LifeSpan Biosciences; Seattle, WA); OVA 257-264 peptide (SIINFEKL) and VSV-NP 52-59 peptide (RGYVYQGL) (CPC Scientific; San Jose, CA).

Techniques: Cell Culture, Irradiation, Enzyme-linked Immunosorbent Assay